MSCEZCult is a specialized medium for umbilical cord blood or bone marrow-derived mesenchymal stem cell expansion.
Different from MSCEZCult Primary, MSCEZCult is not added by GelFix reagent.
MSCEZCult is a serum-free medium. Therefore you do not use trypsin/EDTA during the subculturing process. Deattachment Reagent is recommended instead of trypsin/EDTA.
The medium is added L-glutamine, phenol red, without antibiotic-mycotic, without animal serum. This product contains GMP grade human serum albumin, epidermal growth factor, basic fibroblast growth factors.
Figure 1. The workflow for isolation of umbilical cord blood derived mesenchymal stem cells.
Figure 2. The umbilical cord blood derived mesenchymal stem cells were isolated by MSC EZ Cult.
MSCCult I
MSCCult I was developed for culturing of mesenchymal stem cells (from adipose, umbilical cord, bone marrow). MSCCult I do not contain antibiotic and antimycotic. It is ready-to-use and complete with no additives required.
Enrichment of mesenchymal stem cell proliferation from adipose tissue, umbilical cord tissue, bone marrow tissue for research or production. The medium has been assessed to have a good proliferation effect on mesenchymal stem cells from adipose tissue, umbilical cord tissue and bone marrow tissue.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
MSC EZ Cult
MNC Extraction Kit
MNC Extraction Kit is a kit containing chemicals to isolate mononuclear cells from umbilical cord blood as well as bone marrow.
The products are designed with some solutions to dilute blood, separate mononuclear cells, remove the debris and washing MNCs. The principle of isolation bases on the gradient centrifugation using the Ficoll 1.077.
The products are validated in human bone marrow, and human umbilical cord blood.
ADSCCult I Primary
This is a specialized environment designed for primary culture of adipose derived stem cells from human or animal. The environment does not contain animal serum, is supplemented with antibiotics and antifungal agents, enhances the adhesion factor to help cells easily stick on the surface of the culture equipment. It is ready-to-use and complete with no additives required.
Cultivation of adipose derived stem cells proliferation for research or production. The medium has been shown to have a good proliferation effect on adipose derived stem cells from human and mouse.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the ADSCCult I at 2-8? and protected from light until ready to use.
MSCCult I Primary
MSCCult I Primary is the mesenchymal stem cell culture medium that is optimized for culturing the primary cells isolated from adipose tissue, umbilical cord, bone marrow...
By the MSCCult technology, MSCCult I Primary is enhanced with antibiotic-mycotic to reduce and destroy the bacteria or fungi in the primary samples. Moreover, the MSCCult I Primary also enhanced with some proteins that trigger the adherence of primary cells on the vessel surface.
MSCCult I Primary is tested to culture MNCs isolated from bone marrow, stromal vascular fraction from adipose tissue and umbilical cord tissue.
ADSCCult I
ADSCCult I medium is a specialized medium for adipose derived stem cell culture and expansion. It bases on DMEM/F12 medium that is customized for adipose derived stem cells.
ADSCCult I medium is animal proteins free medium, without fetal bovine serum, without antibiotic-mycotic.
To stilumate the adherence and expansion of ADSCs on vessel surface, ADSCCult I medium is supplemented with platelet rich plasma (GMP grade).
The product is tested on human, rat, murine derived adipose tissue.
The product is suitable for flask culture, and scale-up in bioreactors.
MSC EZ Cult Primary
MSC EZ Cult is the novel technology for umbilical cord blood derived mesenchymal stem cell isolation.
Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Using the novel technology (GelFix*) we can help you isolate MSCs from UCBs easily by few steps.
Firstly, Mononuclear cells (MNCs) should be enriched from umbilical cord blood using MNC Extraction Kit or other commercial kit.
MNCs then are washed twice with Washing Buffer or Phosphate buffer saline before MNCs pellet is re-suspended in MSC EZ Cult Primary.
The cell suspension is plated in the T-75 flask, and incubated in the 5% CO2, 370C for 5 days. After 5 days, the medium is removed and added 10 mL of fresh MSC EZ Cult Primary to T-75 flask. After 5 days, the colonies of MSCs can be observed using the inverted miscroscope. After 12-15 days of incubation, the MSCs can be subcultured and expanded in MSC EZ Cult or MSCCult I.
*GelFix: the MSC EZ Cult medium is gelated after incubated at 370C for 24hrs. The cells need 4-8 hrs to attach on flask surface. After that, gel formation will fix the cells in the flask.
MSCCult MV
MSCCult MV were developed for collecting of mesenchymal stem cell-derived exosomes and microvesicles. MSCCult MV does not contain antibiotics, antimycotic. It is ready-to-use and complete with no additives required.
Usages
MSCCult MV is the medium used to grow mesenchymal stem cells to receive exosomes and microvesicle.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCult MV at 2-8? and protected from light until ready to use.
MSCCult I Basal Medium
MSCCult I Basal Medium is the basal medium of MSCCult I. It can be used when added with MSCCult I Supplement or add 10-15 ?S.
MSCCult I Basala Medium is developed based on the DMEM/F12 formulation with some modifications to reduce ROS, increase the stability.
MSCCult I Supplement 20X
MSCCult I Supplement 20X is the supplement for MSCCult I Basal Medium. After adding MSCCult I Supplement into MSCCult I Basal Medium, we have the completed medium MSCCult I.
MSCCult I Supplement 20X is animal free proteins. It contains human recombinant proteins and platelet lysates (GMP grade).
MSCCult I Kit
MSCCult I Kit is a set of 2 components:
- MSCCult I Basal Medium
- MSCCult I Supplement
When the MSCCult I supplement is added into MSCCult I Basal Medium, it makes the completed medium MSCCult I.