DEATACHMENT were developed for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin/EDTA without protocol changes. It is ready-to-use and complete with no additives required.

Usages

Separate adhering cells from feeding surface. The solution evaluated on mesenchymal stem cells, fibroblasts, keratinocytes, neural stem cells, MCF-7 and HepG2 cancer cells that showed good effect of separating adhesion cells into single cell.

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Washing buffer is a buffer solution commonly used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. We offer a variety of Washing buffer for a range of cell culture applications. Washing buffer are available as Medium (Ready-to-use).

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Transferring Medium is a medium designed to keep the biological samples during the shipping. To maintain the cell viability, the transferring medium is enhanced by HEPES buffer to maintain the pH, nutrients, and antibiotic-mycotic to inhibit the growth of bacteria if any and fungi if any.

This product contains all essential vitamines, amino acids, glucose, pyruvate acid that can help cells or tissues can survive to 48hrs during the shipping at RT or 2-8 oC.

Transferring buffer is a solution used to preserve tissue / samples during transport from the place of collection (hospital) to the laboratory / factory of cell products. The solution is manufactured from materials that meet USP standards, according to GMP-WHO guidelines.

Usages

Transferring buffer is used to preserve tissues such as adipose tissue, umbilical cord tissue, skin tissue, etc. during long-distance transport. The solution enhances cell viability in tissue over long periods of time.

Phosphate Buffer Saline (PBS) is a popular saline in the cell culture. It widely is used to wash cells, tissues during the cells or stem cell processing.

PBS is designed with formulation Ca and Mg free, sterile (autoclave) and ready-to-use.

Deep-Wash Buffer is buffer to wash the adherent cells during monolayer culture. Different to PBS or Washing Buffer, Deep-Wash is enhanced with some enzymes included protease and DNAse that clearly wash proteins as well as other debrises attached in the cell surfaces.

Deep-Wash Buffer is assessed as effiecient method to enhance the cell proliferation because of removal of contact inhibition signals between cells and cells in the monolayer culture.

Using Deep-Wash Buffer to wash cells before adding fresh medium shows significant reduction of cell senescence compared to using PBS or without washing.