Deep-Wash Buffer
Description
Deep-Wash Buffer is buffer to wash the adherent cells during monolayer culture. Different to PBS or Washing Buffer, Deep-Wash is enhanced with some enzymes included protease and DNAse that clearly wash proteins as well as other debrises attached in the cell surfaces.
Deep-Wash Buffer is assessed as effiecient method to enhance the cell proliferation because of removal of contact inhibition signals between cells and cells in the monolayer culture.
Using Deep-Wash Buffer to wash cells before adding fresh medium shows significant reduction of cell senescence compared to using PBS or without washing.
