MSCCult I

MSCCult I is a culture medium that promotes growth of mesenchymal stem cells derived from human umbilical cord tissue, adipose tissue, and bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• Noantibiotics-antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO13485:2016

 

 

MSC EZ Cult

MSCEZCult is a specialized medium for umbilical cord blood or bone marrow-derived mesenchymal stem cell expansion.

Different from MSCEZCult Primary, MSCEZCult is not added by GelFix reagent.

MSCEZCult is a serum-free medium. Therefore you do not use trypsin/EDTA during the subculturing process. Deattachment Reagent is recommended instead of trypsin/EDTA.

The medium is added L-glutamine, phenol red, without antibiotic-mycotic, without animal serum. This product contains GMP grade human serum albumin, epidermal growth factor, basic fibroblast growth factors.

Figure 1. The workflow for isolation of umbilical cord blood derived mesenchymal stem cells.

Figure 2. The umbilical cord blood derived mesenchymal stem cells were isolated by MSC EZ Cult.

 

MNC Extraction Kit

MNC Extraction Kit is used to isolate mononuclear cells from umbilical cord and bone marrow.

The product contains various solutions to dilute blood, separate mononuclear cells, remove the debris, and washing them. The principle of isolation is based on the gradient centrifugation using the Ficoll 1.077.

 

ADSCCult I Primary

ADSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from human adipose tissue.

• Completed medium

• With antibiotics - antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO 13485:2016

MSCCult I Primary

MSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from solid tissues such as human umbilical cord tissue, adipose tissue, bone marrow, etc.

• Completed medium

• With antibiotics - antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO 13485:2016

ADSCCult I

ADSCCult I is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.

• Completed medium

• Noantibiotics-antimycotics

• Coating is NOT required

• Quality and traceability guaranteeby ISO13485:2016

MSC EZ Cult Primary

MSC EZ Cult is the novel technology for umbilical cord blood derived mesenchymal stem cell isolation.

Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Using the novel technology (GelFix*) we can help you isolate MSCs from UCBs easily by few steps.

Firstly, Mononuclear cells (MNCs) should be enriched from umbilical cord blood using MNC Extraction Kit or other commercial kit.

MNCs then are washed twice with Washing Buffer or Phosphate buffer saline before MNCs pellet is re-suspended in MSC EZ Cult Primary.

The cell suspension is plated in the T-75 flask, and incubated in the 5% CO2, 370C for 5 days. After 5 days, the medium is removed and added 10 mL of fresh MSC EZ Cult Primary to T-75 flask. After 5 days, the colonies of MSCs can be observed using the inverted miscroscope. After 12-15 days of incubation, the MSCs can be subcultured and expanded in MSC EZ Cult or MSCCult I.

*GelFix: the MSC EZ Cult medium is gelated after incubated at 370C for 24hrs. The cells need 4-8 hrs to attach on flask surface. After that, gel formation will fix the cells in the flask.

 

MSCCult MV

MSCCult MV were developed for  collecting of mesenchymal stem cell-derived exosomes and microvesicles.  MSCCult MV does not contain antibiotics, antimycotic. It is ready-to-use and complete with no additives required.

Usages

MSCCult MV is the medium used to grow mesenchymal stem cells to receive exosomes and microvesicle.

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the MSCCult MV at 2-8? and protected from light until ready to use.

MSCCult I Basal Medium

MSCCult I Basal Medium is the basal medium of MSCCult I. It can be used when added with MSCCult I Supplement or add 10-15 ?S.

MSCCult I Basala Medium is developed based on the DMEM/F12 formulation with some modifications to reduce ROS, increase the stability.

MSCCult I Supplement 20X

MSCCult I Supplement 20X is the supplement for MSCCult I Basal Medium. After adding MSCCult I Supplement into MSCCult I Basal Medium, we have the completed medium MSCCult I.

MSCCult I Supplement 20X is animal free proteins. It contains human recombinant proteins and platelet lysates (GMP grade).

MSCCult I Kit

MSCCult I Kit is a set of 2 components:

  • MSCCult I Basal Medium
  • MSCCult I Supplement

When the MSCCult I supplement is added into MSCCult I Basal Medium, it makes the completed medium MSCCult I.

Cryosave I

Cryosave I is supplied in a 1X format for the cryopreservation of cells and mesenchymal stem cells. It contains 10X DMSO and does not use any animal-derived components. The product also contains phenol red. 

• Completed medium 1X

• No antibiotics - antimycotics

• No animal derived protein

• Quality and traceability guarantee by ISO 13485:2016

Cryosave II

Crosave II is supplied in a 1X format for the cryopreservation of cells and mesenchymal stem cells. It contains 5% DMSO and is manufactured with a special formula that includes all components that meet USP standards.
• Completed medium 1X
• No antibiotics - antimycotics
• No animal derived protein
• No phenol red
• Quality and traceability guarantee by ISO 13485:2016.

ADSCCult II

ADSCCult II is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCult II

MSCCult II is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord tissue. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics - antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO 13485:2016.

 

BMMSCCult

BMMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics 

• Coating is NOT required

• With high-stable growth factors

• Quality and trace ability guarantee by ISO13485:2016

DentalCult

DentalCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCryosave OTS TH

MSCCryosave OTS TH is supplied in a 1X format for the cryopreservation of Mesenchymal stem cells. It contains human serum albumin, which meets USP standards, and trehalose.
• Completed medium 1X
• No antibiotics - antimycotics
• No animal derived protein
• No phenol red
• No cryoprotectant (DMSO, glycerol...)
• Quality and traceability guarantee by ISO 13485:2016

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the MSCCryosave OTS  at 2-8°C and protected from light until ready to use.

Usages

MSCCryosave OTS is well suited for the production of off-the-shelf stem cell medicine. The product has been assessed for its effectiveness in cryopreservation – of mesenchymal stem cells from adipose tissue and human umbilical cord – for good cryopreservation efficiency with a high percentage of viable cells after defrosting.

 

UCBMSCCult

UCBMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord blood. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCult OTS

MSCCult OTS is a product with an advanced formula that does not use phenol red or any animal-derived protein. It has low endotoxin and is suitable for promoting the growth of mesenchymal stem cells from different tissue types.

• Completed medium

• No antibiotics - antimycotics

• Coating is NOT required

• With high-stable growth factors

• Stem cell drug production pipelines

• Quality and traceability guarantee by ISO 13485:2016

 

MSCCult OTS MV

MSCCult OTS MV with low toxicity, it is suitable for producing conditioned medium, extracellular microvesicle, and exosomes from various types of mesenchymal stem cells.

• Completed medium

• No antibiotics - antimycotics

• No phenol red

• No animal-derived protein

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO 13485:2016.

MSCCult CD

MSCCult CD is a product manufactured without any attachment factors and requires surface coating before cell seeding.

? Completed medium

• No antibiotics - antimycotics

• No phenol red

• No animal-derived protein

• With high-stable growth factors

• Coating required before seeding

• Quality and traceability guarantee by ISO 13485:2016.