MSCEZCult is a specialized medium for umbilical cord blood or bone marrow-derived mesenchymal stem cell expansion.
Different from MSCEZCult Primary, MSCEZCult is not added by GelFix reagent.
MSCEZCult is a serum-free medium. Therefore you do not use trypsin/EDTA during the subculturing process. Deattachment Reagent is recommended instead of trypsin/EDTA.
The medium is added L-glutamine, phenol red, without antibiotic-mycotic, without animal serum. This product contains GMP grade human serum albumin, epidermal growth factor, basic fibroblast growth factors.
Figure 1. The workflow for isolation of umbilical cord blood derived mesenchymal stem cells.
Figure 2. The umbilical cord blood derived mesenchymal stem cells were isolated by MSC EZ Cult.
MSCCult I
MSCCult I is a culture medium that promotes growth of mesenchymal stem cells derived from human umbilical cord tissue, adipose tissue, and bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• Noantibiotics-antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO13485:2016
MSC EZ Cult
MNC Extraction Kit
MNC Extraction Kit is used to isolate mononuclear cells from umbilical cord and bone marrow.
The product contains various solutions to dilute blood, separate mononuclear cells, remove the debris, and washing them. The principle of isolation is based on the gradient centrifugation using the Ficoll 1.077.
ADSCCult I Primary
ADSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from human adipose tissue.
• Completed medium
• With antibiotics - antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016
MSCCult I Primary
MSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from solid tissues such as human umbilical cord tissue, adipose tissue, bone marrow, etc.
• Completed medium
• With antibiotics - antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016
ADSCCult I
ADSCCult I is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.
• Completed medium
• Noantibiotics-antimycotics
• Coating is NOT required
• Quality and traceability guaranteeby ISO13485:2016
MSC EZ Cult Primary
MSC EZ Cult is the novel technology for umbilical cord blood derived mesenchymal stem cell isolation.
Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Using the novel technology (GelFix*) we can help you isolate MSCs from UCBs easily by few steps.
Firstly, Mononuclear cells (MNCs) should be enriched from umbilical cord blood using MNC Extraction Kit or other commercial kit.
MNCs then are washed twice with Washing Buffer or Phosphate buffer saline before MNCs pellet is re-suspended in MSC EZ Cult Primary.
The cell suspension is plated in the T-75 flask, and incubated in the 5% CO2, 370C for 5 days. After 5 days, the medium is removed and added 10 mL of fresh MSC EZ Cult Primary to T-75 flask. After 5 days, the colonies of MSCs can be observed using the inverted miscroscope. After 12-15 days of incubation, the MSCs can be subcultured and expanded in MSC EZ Cult or MSCCult I.
*GelFix: the MSC EZ Cult medium is gelated after incubated at 370C for 24hrs. The cells need 4-8 hrs to attach on flask surface. After that, gel formation will fix the cells in the flask.
MSCCult MV
MSCCult MV were developed for collecting of mesenchymal stem cell-derived exosomes and microvesicles. MSCCult MV does not contain antibiotics, antimycotic. It is ready-to-use and complete with no additives required.
Usages
MSCCult MV is the medium used to grow mesenchymal stem cells to receive exosomes and microvesicle.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCult MV at 2-8? and protected from light until ready to use.
MSCCult I Basal Medium
MSCCult I Basal Medium is the basal medium of MSCCult I. It can be used when added with MSCCult I Supplement or add 10-15 ?S.
MSCCult I Basala Medium is developed based on the DMEM/F12 formulation with some modifications to reduce ROS, increase the stability.
MSCCult I Supplement 20X
MSCCult I Supplement 20X is the supplement for MSCCult I Basal Medium. After adding MSCCult I Supplement into MSCCult I Basal Medium, we have the completed medium MSCCult I.
MSCCult I Supplement 20X is animal free proteins. It contains human recombinant proteins and platelet lysates (GMP grade).
MSCCult I Kit
MSCCult I Kit is a set of 2 components:
- MSCCult I Basal Medium
- MSCCult I Supplement
When the MSCCult I supplement is added into MSCCult I Basal Medium, it makes the completed medium MSCCult I.
Cryosave I
Cryosave I is supplied in a 1X concentration for the cryopreservation of cells and mesenchymal stem cells. It contains 10X DMSO concentration, phenol red and no animal-derived components.
• Completed medium 1X concentration
• Antibiotics-free
• Antimycotics-free
• Animal component-free
• Quality and traceability guarantee by ISO 13485:2016
Cryosave II
Cryosave II is supplied in a 1X concentration for the cryopreservation of cells and mesenchymal stem cells. It is a ready-to-use, complete, and uniquely formulated animal component-free, phenol red-free cryopreservation medium containing 5% DMSO and all components that meet USP standards.
• Completed medium 1X concentration
• Antibiotics-free
• Antimycotics-free
• Animal component-free
• Phenol red-free
• Quality and traceability guarantee by ISO 13485:2016
ADSCCult II
ADSCCult II is a culture medium used to promote the growth of mesenchymal stem cells derived from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self-renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCult II
MSCCult II is a culture medium that promotes the growth of mesenchymal stem cells derived from human umbilical cord tissue. Formulated with unique ingredients, MSCCult II medium induces cellular self-renewal, yet inhibits differentiation, and prevents cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO 13485:2016.
BMMSCCult
BMMSCCult is a culture medium that promotes the growth of mesenchymal stem cells derived from human bone marrow. Formulated with unique ingridents, BMMSCCult medium induces cellular self-renewal, yet inhibits differentiation, and prevents cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and trace ability guarantee by ISO13485:2016
DentalCult
DentalCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• No antibiotics-antimycotics
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCryosave OTS TH
MSCCryosave OTS TH is supplied in a 1X concentration for cryopreservation of Mesenchymal stem cells. It contains human serum albumin, which meets USP standards, and trehalose.
• Completed medium 1X
• Antibiotic-free
• Antimycotic-free
• Animal-component free
• Phenol red-free
• Cryoprotectant-free (DMSO, glycerol...)
• Quality and traceability guarantee by ISO 13485:2016
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCryosave OTS at 2-8°C and away from light until ready to use.
Usages
MSCCryosave OTS is a off-the-shelf cryopreservation medium. The product shows its efficiency in cryopreservation of mesenchymal stem cells derived from adipose tissue and human umbilical cord. Moreover, MSCCryosave OTS maintains a high percentage of viable cells after thawing.
UCBMSCCult
UCBMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord blood. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• No antibiotics-antimycotics
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCult OTS
MSCCult OTS is a complete, ready-to-use, advance and uniquely formulated antibiotic-free, antimycotic-free, animal component-free, phenol red-free, and defined culture medium containing low endotoxin. MSCCult OTS promotes cell growth in mesenchymal stem cell derived from various tissue types.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Stem cell drug production pipelines
• Quality and traceability guarantee by ISO 13485:2016
MSCCult OTS MV
MSCCult OTS MV is uniquely antibiotic-free, antimycotic-free, phenol red-free, animal component-free and completed medium with low toxicity. MSCCult OTS MV is designed to culture extracellular micro-vesicle and exosomes from various types of mesenchymal stem cells.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Phenol red-free
• Animal component-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO 13485:2016.
MSCCult CD
MSCCult CD is a manufactured culture medium contains no attachment factors and requires surface coating before cell seeding.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Phenol red-free
• Animal component-free
• With high-stable growth factors
• Coating required before seeding
• Quality and traceability guarantee by ISO 13485:2016.