MSCEZCult is a specialized medium for umbilical cord blood or bone marrow-derived mesenchymal stem cell expansion.
Different from MSCEZCult Primary, MSCEZCult is not added by GelFix reagent.
MSCEZCult is a serum-free medium. Therefore you do not use trypsin/EDTA during the subculturing process. Deattachment Reagent is recommended instead of trypsin/EDTA.
The medium is added L-glutamine, phenol red, without antibiotic-mycotic, without animal serum. This product contains GMP grade human serum albumin, epidermal growth factor, basic fibroblast growth factors.
Figure 1. The workflow for isolation of umbilical cord blood derived mesenchymal stem cells.
Figure 2. The umbilical cord blood derived mesenchymal stem cells were isolated by MSC EZ Cult.
Cell Extraction Kit
Cell Extraction Kit can be used to extract single cells from solid tissues (up to 50 grams of tissue) including adipose tissues, umbilical cords, placenta, muscle, etc.
The product was confirmed for its high efficacy in extracting single cells from adipose tissues and umbilical cord tissues.
The product uses a combination of combitorial mechanisms of enzymes and machenic forces to break up the solid tissues to single cells.
The enzyme cocktails are specially designed with high-puried collagenase with dispase.
Quality and traceability guarantee by ISO 13485:2016.
MSCCult I
MSCCult I is a culture medium that promotes growth of mesenchymal stem cells derived from human umbilical cord tissue, adipose tissue, and bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• Noantibiotics-antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO13485:2016
MSC EZ Cult
MNC Extraction Kit
MNC Extraction Kit is used to isolate mononuclear cells from umbilical cord and bone marrow.
The product contains various solutions to dilute blood, separate mononuclear cells, remove the debris, and washing them. The principle of isolation is based on the gradient centrifugation using the Ficoll 1.077.
ADSCCult I Primary
ADSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from human adipose tissue.
• Completed medium
• With antibiotics - antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016
MNC Extraction Easy Kit
MNC Extraction Easy Kit uses a simple, high efficacy, and reproducible procedure to isolate the mononuclear cells (MNCs) from a blood sample obtained from umbilical cord and bone marrow.
The new advance designed porous gel is put in the separation tubes to isolate the MNCs like immune cells and red blood cells from blood sample.
MSCCult I Primary
MSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from solid tissues such as human umbilical cord tissue, adipose tissue, bone marrow, etc.
• Completed medium
• With antibiotics - antimycotics
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016
ADSCCult I
ADSCCult I is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.
• Completed medium
• Noantibiotics-antimycotics
• Coating is NOT required
• Quality and traceability guaranteeby ISO13485:2016
MSC EZ Cult Primary
MSC EZ Cult is the novel technology for umbilical cord blood derived mesenchymal stem cell isolation.
Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Using the novel technology (GelFix*) we can help you isolate MSCs from UCBs easily by few steps.
Firstly, Mononuclear cells (MNCs) should be enriched from umbilical cord blood using MNC Extraction Kit or other commercial kit.
MNCs then are washed twice with Washing Buffer or Phosphate buffer saline before MNCs pellet is re-suspended in MSC EZ Cult Primary.
The cell suspension is plated in the T-75 flask, and incubated in the 5% CO2, 370C for 5 days. After 5 days, the medium is removed and added 10 mL of fresh MSC EZ Cult Primary to T-75 flask. After 5 days, the colonies of MSCs can be observed using the inverted miscroscope. After 12-15 days of incubation, the MSCs can be subcultured and expanded in MSC EZ Cult or MSCCult I.
*GelFix: the MSC EZ Cult medium is gelated after incubated at 370C for 24hrs. The cells need 4-8 hrs to attach on flask surface. After that, gel formation will fix the cells in the flask.
Cell Extraction EZ Kit
Cell Extraction EZ kit is a new technology to dissociate solid tissues (adipose tissue, umbilical cord, and placenta) or tumors (breast tumor) into single cells. Unlike Cell Extraction Kit, the enzymes (collagenase and dispase) of Cell Extraction EZ kit are coated in the extractor. Hence, the product is easier to use and to transport.
Deattachment
DEATACHMENT were developed for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin/EDTA without protocol changes. It is ready-to-use and complete with no additives required.
Usages
Separate adhering cells from feeding surface. The solution evaluated on mesenchymal stem cells, fibroblasts, keratinocytes, neural stem cells, MCF-7 and HepG2 cancer cells that showed good effect of separating adhesion cells into single cell.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
MSCCult MV
MSCCult MV were developed for collecting of mesenchymal stem cell-derived exosomes and microvesicles. MSCCult MV does not contain antibiotics, antimycotic. It is ready-to-use and complete with no additives required.
Usages
MSCCult MV is the medium used to grow mesenchymal stem cells to receive exosomes and microvesicle.
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCult MV at 2-8? and protected from light until ready to use.
EZ Dropper
EZ Dropper is a sterile plastic container for cosmetic products. The container can be easily sealed before filled by liquid. After sealed this product can maintain the sterility for a long-time.
The EZ Dropper can be storaged in 2-8 oC for a long time.
Washing Buffer
Washing buffer is a ready-to-use buffer as well as short-term medium for various cell culture applications, such as washing cells, transporting cells, diluting cells for counting, and preparing reagents.
The products are for in vitro use only and are not for diagnostic or therapeutic procedures.
Transferring Medium
Transferring Medium is a medium designed to keep the biological samples during transporting. To maintain the cell viability, the transferring medium is enhanced by HEPES buffer to maintain the pH, nutrients, and antibiotic-mycotic to inhibit the growth of bacteria if any and fungi if any. Due to containing HEPES, Transferring Medium maintains cell viability well with stable pH level, nutrients, and antibiotic-mycotic to prevent bacterial growth and fungal growth.
This product contains all essential vitamins, amino acids, glucose, pyruvate acid that improve viability of cells or tissues up to 48hrs during transporting from 2oC to RT
Transferring buffer solution is used to preserve tissue / samples during transport from the place of collection (hospital) to the laboratory / factory of cell products. The solution is manufactured from materials that meet USP standards, according to GMP-WHO guidelines.
Usages
Transferring buffer is used to preserve tissues such as adipose tissue, umbilical cord tissue, skin tissue, etc. during long-distance transport. The solution enhances cell viability in tissue over long periods of time.
MSCCult I Basal Medium
MSCCult I Basal Medium is the basal medium of MSCCult I. It can be used when added with MSCCult I Supplement or add 10-15 ?S.
MSCCult I Basala Medium is developed based on the DMEM/F12 formulation with some modifications to reduce ROS, increase the stability.
MSCCult I Supplement 20X
MSCCult I Supplement 20X is the supplement for MSCCult I Basal Medium. After adding MSCCult I Supplement into MSCCult I Basal Medium, we have the completed medium MSCCult I.
MSCCult I Supplement 20X is animal free proteins. It contains human recombinant proteins and platelet lysates (GMP grade).
MSCCult I Kit
MSCCult I Kit is a set of 2 components:
- MSCCult I Basal Medium
- MSCCult I Supplement
When the MSCCult I supplement is added into MSCCult I Basal Medium, it makes the completed medium MSCCult I.
Phosphate Buffer Saline (PBS)
Phosphate Buffer Saline (PBS) is a popular and widely used for washing cells or tissues in the cell culture.
PBS is a sterile ready-to-use product that contains Ca and Mg free, sterile (autoclave)
Deep-Wash Buffer
Deep-Wash Buffer solution is used for cell washing for various cell culture applications. Compared to PBS or Washing Buffer, Deep-Wash Buffer is enhanced due to containing some enzymes, including protease and DNase. The enhancement can help washing-off proteins as well as other debris attached on the cell surfaces.
Deep-Wash Buffer shows efficaciuos ability to enhance cell proliferation due to the removal of contact inhibition cell-to-cell signals in monolayer culture.
Deep-Wash Buffer also shows significant reduction of cell senescence after washing and before adding culture medium compared to using PBS or without washing.
ExoCryosave OTS
ExoCryosave OTS is used for exosomes cryopreservation and meets the standards for human use. It is supplied in 2X concentration and contains human serum albumin, which meets USP standards, and trehalose.
? Completed medium 2X
? Antibiotics-free
? Antimycotics-free
? Animal-free
? Phenol red-free
? Cryoprotectant-free (DMSO, glycerol...)
? Quality and traceability guarantee by ISO 13485:2016
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store ExoCryosave OTS at 2-8oC and away from light until ready to use.
Usages
ExoCryosave OTS is a off-the-shelf cryopreservation medium. The product shows its efficiency in cryopreservation of 2 types of mesenchymal stem cells like adipose tissue and human umbilical cord. Moreover, ExoCryosave OTS maintains a high percentage of viable cells after thawing.
Cryosave I
Cryosave I is supplied in a 1X concentration for the cryopreservation of cells and mesenchymal stem cells. It contains 10X DMSO concentration, phenol red and no animal-derived components.
• Completed medium 1X concentration
• Antibiotics-free
• Antimycotics-free
• Animal component-free
• Quality and traceability guarantee by ISO 13485:2016
Cryosave II
Cryosave II is supplied in a 1X concentration for the cryopreservation of cells and mesenchymal stem cells. It is a ready-to-use, complete, and uniquely formulated animal component-free, phenol red-free cryopreservation medium containing 5% DMSO and all components that meet USP standards.
• Completed medium 1X concentration
• Antibiotics-free
• Antimycotics-free
• Animal component-free
• Phenol red-free
• Quality and traceability guarantee by ISO 13485:2016
MSCCryosave OTS
MSCCryosave OTS is supplied in a 1X concentration for the cryopreservation of cells and mesenchymal stem cells. It is a uniquely formulated animal component-free, cryoprotectant-free, phenol red-free, and defined cryopresevation medium containing human serum albumin, which is approved by USP standards.
• Completed medium 1X concentration
• Antibiotics-free
• Antimycotics-free
• Animal component-free
• Cryoprotectant-free
• Phenol red-free
• Quality and traceability guarantee by ISO 13485:2016
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCryosave OTS at 2-8oC and away from light until ready to use.
Usages
MSCCryosave OTS is a off-the-shelf cryopreservation medium. The product shows its efficiency in cryopreservation of 2 types of mesenchymal stem cells like adipose tissue and human umbilical cord. Moreover, MSCCryosave OTS maintains a high percentage of viable cells after thawing.
ThawBest
Designed to reduce shock/stress due to sudden decrease of osmolarity, Thawbest is recommended for thawing all types of cryopreserved cells while preserving high cell viability. It should be used for 2:1 ratio, meaning 2 volumes of Thawbest to 1 volume of cell suspension.
AfterFreeze
AfterFreeze is an off-the-shelf solution used for thawing cryopreservation mesenchymal stem cell while preserving higher viability of the cells compared to physiological saline solution. AfterFreeze contains all components that are approved by USP standards and GMP-WHO guidelines.
Usages
Use during the thawing process of cryopreservation cells. The product was evaluated on mesenchymal stem cell lines from human adipose tissue and umbilical cord.
Cell Extraction Mini Kit
Cell Extraction Mini Kit is a set of reagents and consumables used to extract single cells from solid tissues (up to 10 grams of tissue) such as adipose tissue, umbilical cord,placenta, muscle, etc.
The product shows outstanding results for single cells collection from adipose tissues and umbilical cord tissues.
Designed to yield the best extraction results, this product relies on the combitorial mechanisms of enzymes and mechanical forces to break down the solid tissues to single cells.
The enzyme cocktails are specially desinged with high-puried collagenase with dispase.
Quality and traceability guarantee by ISO 13485:2016.
ADSCCult II
ADSCCult II is a culture medium used to promote the growth of mesenchymal stem cells derived from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self-renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCult II
MSCCult II is a culture medium that promotes the growth of mesenchymal stem cells derived from human umbilical cord tissue. Formulated with unique ingredients, MSCCult II medium induces cellular self-renewal, yet inhibits differentiation, and prevents cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO 13485:2016.
BMMSCCult
BMMSCCult is a culture medium that promotes the growth of mesenchymal stem cells derived from human bone marrow. Formulated with unique ingridents, BMMSCCult medium induces cellular self-renewal, yet inhibits differentiation, and prevents cell aging during in vitro expansion.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Quality and trace ability guarantee by ISO13485:2016
DentalCult
DentalCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• No antibiotics-antimycotics
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCryosave OTS TH
MSCCryosave OTS TH is supplied in a 1X concentration for cryopreservation of Mesenchymal stem cells. It contains human serum albumin, which meets USP standards, and trehalose.
• Completed medium 1X
• Antibiotic-free
• Antimycotic-free
• Animal-component free
• Phenol red-free
• Cryoprotectant-free (DMSO, glycerol...)
• Quality and traceability guarantee by ISO 13485:2016
The products are for in vitro use only and not for diagnostic or therapeutic procedures.
Store the MSCCryosave OTS at 2-8°C and away from light until ready to use.
Usages
MSCCryosave OTS is a off-the-shelf cryopreservation medium. The product shows its efficiency in cryopreservation of mesenchymal stem cells derived from adipose tissue and human umbilical cord. Moreover, MSCCryosave OTS maintains a high percentage of viable cells after thawing.
UCBMSCCult
UCBMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord blood. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.
• Completed medium
• No antibiotics-antimycotics
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO13485:2016.
MSCCult OTS
MSCCult OTS is a complete, ready-to-use, advance and uniquely formulated antibiotic-free, antimycotic-free, animal component-free, phenol red-free, and defined culture medium containing low endotoxin. MSCCult OTS promotes cell growth in mesenchymal stem cell derived from various tissue types.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• With high-stable growth factors
• Stem cell drug production pipelines
• Quality and traceability guarantee by ISO 13485:2016
MSCCult OTS MV
MSCCult OTS MV is uniquely antibiotic-free, antimycotic-free, phenol red-free, animal component-free and completed medium with low toxicity. MSCCult OTS MV is designed to culture extracellular micro-vesicle and exosomes from various types of mesenchymal stem cells.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Phenol red-free
• Animal component-free
• Coating is NOT required
• With high-stable growth factors
• Quality and traceability guarantee by ISO 13485:2016.
MSCCult CD
MSCCult CD is a manufactured culture medium contains no attachment factors and requires surface coating before cell seeding.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Phenol red-free
• Animal component-free
• With high-stable growth factors
• Coating required before seeding
• Quality and traceability guarantee by ISO 13485:2016.
ImmunoAdd
ImmunoAdd is uniquely formulated antibiotic-free, antimycotic-free, and completed medium containing components that significantly strengthen the immune system of mesenchymal stem cells up to 48 hours after treatment.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016.
SecretoAdd
SecretoAdd enhances the secretion ability of mesenchymal stem cells. The product is produced in a 100X concentration, and it can be directly added to the culture medium. After 48 hours of culture under low oxygen conditions (5-10%), the amount of secretion significantly increases.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016.
MSCCult Muse
MSCCult Muse is a completed and ready-to-use growth culture medium for the population of SSEA-3+CD105+ muse cells.
• Completed medium
• Antibiotic-free
• Antimycotic-free
• Coating is NOT required
• Quality and traceability guarantee by ISO 13485:2016.
Trypsinizer
Trypsinizer is used to detach mesenchymal stem cells adhered on culture flasks or dishes. It utilizes a combination of plant-derived trypsin enzymes.
- Completed medium
- Antibiotic-free
- Antimycotic-free
- Animal component-free
- Phenol red-free
- Quality and traceability guarantee by ISO 13485:2016.
Deattachment Advanced
Deattachment Advanced is used to detach mesenchymal stem cells adhered on flasks or in bioreactors. It utilizes a combination of recombinant trypsin and collagenase.
- Completed medium
- Antibiotic-free
- Antimycotic-free
- Animal component-free
- Phenol red-free
- Quality and traceability guarantee by ISO 13485:2016
Phosphate Buffer Saline (PBS) OTS
PBS OTS is uniquily formulated antibiotic-free, antimycotic-free, animal component-free, phenol red-free, and completed saline solution used for cell washing with low endotoxin levels; hence, making it suitable for the production of therapeutic
cells.
- Completed medium
- Antibiotic-free
- Antimycotic-free
- Animal component-free
- Phenol red-free
- Low endotoxin
- Quality and traceability guarantee by ISO 13485:2016