Cell Extraction Kit

Cell Extraction Kit can be used to extract single cells from solid tissues (up to 50 grams of tissue) including adipose tissues, umbilical cords, placenta, muscle, etc.

The product was confirmed for its high efficacy in extracting single cells from adipose tissues and umbilical cord tissues.

The product uses a combination of combitorial mechanisms of enzymes and machenic forces to break up the solid tissues to single cells.

The enzyme cocktails are specially designed with high-puried collagenase with dispase.

 Quality and traceability guarantee by ISO 13485:2016.

MSCCult I

MSCCult I is a culture medium that promotes growth of mesenchymal stem cells derived from human umbilical cord tissue, adipose tissue, and bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• Noantibiotics-antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO13485:2016

 

 

MSC EZ Cult

MSCEZCult is a specialized medium for umbilical cord blood or bone marrow-derived mesenchymal stem cell expansion.

Different from MSCEZCult Primary, MSCEZCult is not added by GelFix reagent.

MSCEZCult is a serum-free medium. Therefore you do not use trypsin/EDTA during the subculturing process. Deattachment Reagent is recommended instead of trypsin/EDTA.

The medium is added L-glutamine, phenol red, without antibiotic-mycotic, without animal serum. This product contains GMP grade human serum albumin, epidermal growth factor, basic fibroblast growth factors.

Figure 1. The workflow for isolation of umbilical cord blood derived mesenchymal stem cells.

Figure 2. The umbilical cord blood derived mesenchymal stem cells were isolated by MSC EZ Cult.

 

MNC Extraction Kit

MNC Extraction Kit is used to isolate mononuclear cells from umbilical cord and bone marrow.

The product contains various solutions to dilute blood, separate mononuclear cells, remove the debris, and washing them. The principle of isolation is based on the gradient centrifugation using the Ficoll 1.077.

 

ADSCCult I Primary

ADSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from human adipose tissue.

• Completed medium

• With antibiotics - antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO 13485:2016

MNC Extraction Easy Kit

MNC Extraction Easy Kit uses a simple, high efficacy, and reproducible procedure to isolate the mononuclear cells (MNCs) from a blood sample obtained from umbilical cord and bone marrow.

The new advance designed porous gel is put in the separation tubes to isolate the MNCs like immune cells and red blood cells from blood sample.

 

MSCCult I Primary

MSCCult I Primary is used for primary culture of mesenchymal stem cells obtained from solid tissues such as human umbilical cord tissue, adipose tissue, bone marrow, etc.

• Completed medium

• With antibiotics - antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO 13485:2016

ADSCCult I

ADSCCult I is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.

• Completed medium

• Noantibiotics-antimycotics

• Coating is NOT required

• Quality and traceability guaranteeby ISO13485:2016

MSC EZ Cult Primary

MSC EZ Cult is the novel technology for umbilical cord blood derived mesenchymal stem cell isolation.

Umbilical cord blood (UCB) is a rich source of stem cells; nonetheless, isolation of mesenchymal stem cells (MSCs) from UCB presents technical challenges. Using the novel technology (GelFix*) we can help you isolate MSCs from UCBs easily by few steps.

Firstly, Mononuclear cells (MNCs) should be enriched from umbilical cord blood using MNC Extraction Kit or other commercial kit.

MNCs then are washed twice with Washing Buffer or Phosphate buffer saline before MNCs pellet is re-suspended in MSC EZ Cult Primary.

The cell suspension is plated in the T-75 flask, and incubated in the 5% CO2, 370C for 5 days. After 5 days, the medium is removed and added 10 mL of fresh MSC EZ Cult Primary to T-75 flask. After 5 days, the colonies of MSCs can be observed using the inverted miscroscope. After 12-15 days of incubation, the MSCs can be subcultured and expanded in MSC EZ Cult or MSCCult I.

*GelFix: the MSC EZ Cult medium is gelated after incubated at 370C for 24hrs. The cells need 4-8 hrs to attach on flask surface. After that, gel formation will fix the cells in the flask.

 

Cell Extraction EZ Kit

Cell Extraction EZ kit is a new technology to dissociate solid tissues (adipose tissue, umbilical cord, and placenta) or tumors (breast tumor) into single cells. Unlike Cell Extraction Kit, the enzymes (collagenase and dispase) of Cell Extraction EZ kit are coated in the extractor. Hence, the product is easier to use and to transport.

 

Deattachment

DEATACHMENT were developed for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin/EDTA without protocol changes. It is ready-to-use and complete with no additives required.

Usages

Separate adhering cells from feeding surface. The solution evaluated on mesenchymal stem cells, fibroblasts, keratinocytes, neural stem cells, MCF-7 and HepG2 cancer cells that showed good effect of separating adhesion cells into single cell.

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

 

MSCCult MV

MSCCult MV were developed for  collecting of mesenchymal stem cell-derived exosomes and microvesicles.  MSCCult MV does not contain antibiotics, antimycotic. It is ready-to-use and complete with no additives required.

Usages

MSCCult MV is the medium used to grow mesenchymal stem cells to receive exosomes and microvesicle.

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the MSCCult MV at 2-8? and protected from light until ready to use.

EZ Dropper

EZ Dropper is a sterile plastic container for cosmetic products. The container can be easily sealed before filled by liquid. After sealed this product can maintain the sterility for a long-time.

The EZ Dropper can be storaged in 2-8 oC for a long time.

Washing Buffer

Washing buffer is a ready-to-use buffer as well as short-term medium for various cell culture applications, such as washing cells, transporting cells, diluting cells for counting, and preparing reagents. 

The products are for in vitro use only and are not for diagnostic or therapeutic procedures.

 

Transferring Medium

Transferring Medium is a medium designed to keep the biological samples during transporting. To maintain the cell viability, the transferring medium is enhanced by HEPES buffer to maintain the pH, nutrients, and antibiotic-mycotic to inhibit the growth of bacteria if any and fungi if any. Due to containing HEPES, Transferring Medium maintains cell viability well with stable pH level, nutrients, and antibiotic-mycotic to prevent bacterial growth and fungal growth.

This product contains all essential vitamins, amino acids, glucose, pyruvate acid that improve viability of cells or tissues up to 48hrs during transporting from 2oC to RT

Transferring buffer solution is used to preserve tissue / samples during transport from the place of collection (hospital) to the laboratory / factory of cell products. The solution is manufactured from materials that meet USP standards, according to GMP-WHO guidelines.

Usages

Transferring buffer is used to preserve tissues such as adipose tissue, umbilical cord tissue, skin tissue, etc. during long-distance transport. The solution enhances cell viability in tissue over long periods of time.

 

MSCCult I Basal Medium

MSCCult I Basal Medium is the basal medium of MSCCult I. It can be used when added with MSCCult I Supplement or add 10-15 ?S.

MSCCult I Basala Medium is developed based on the DMEM/F12 formulation with some modifications to reduce ROS, increase the stability.

MSCCult I Supplement 20X

MSCCult I Supplement 20X is the supplement for MSCCult I Basal Medium. After adding MSCCult I Supplement into MSCCult I Basal Medium, we have the completed medium MSCCult I.

MSCCult I Supplement 20X is animal free proteins. It contains human recombinant proteins and platelet lysates (GMP grade).

MSCCult I Kit

MSCCult I Kit is a set of 2 components:

  • MSCCult I Basal Medium
  • MSCCult I Supplement

When the MSCCult I supplement is added into MSCCult I Basal Medium, it makes the completed medium MSCCult I.

Phosphate Buffer Saline (PBS)

Phosphate Buffer Saline (PBS) is a popular and widely used for washing cells or tissues in the cell culture.

PBS is a sterile ready-to-use product that contains Ca and Mg free, sterile (autoclave)

 

Deep-Wash Buffer

Deep-Wash Buffer is buffer to wash the adherent cells during monolayer culture. Different to PBS or Washing Buffer, Deep-Wash is enhanced with some enzymes included protease and DNAse that clearly wash proteins as well as other debrises attached in the cell surfaces.

Deep-Wash Buffer is assessed as effiecient method to enhance the cell proliferation because of removal of contact inhibition signals between cells and cells in the monolayer culture.

Using Deep-Wash Buffer to wash cells before adding fresh medium shows significant reduction of cell senescence compared to using PBS or without washing.

 

ExoCryosave OTS

ExoCryosave OTS is used for freezing and storing exosomes and meets the standards for use in humans. It is supplied  in a 2X format and contains human serum albumin, which meets USP standards, and trehalose.

? Completed medium 2X
? No antibiotics - antimycotics
? No animal derived protein
? No phenol red
? No cryoprotectant (DMSO, glycerol...)
? Quality and traceability guarantee by ISO 13485:2016

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the ExoCryosave OTS at 2-8? and protected from light until ready to use.

Usages

ExoCryosave OTS is well suited for the production of off-the-shelf stem cell medicine. The product has been assessed for its effectiveness in cryopreservation – of mesenchymal stem cells from adipose tissue and human umbilical cord – for good cryopreservation efficiency with a high percentage of viable cells after defrosting.

Cryosave I

Cryosave I is supplied in a 1X format for the cryopreservation of cells and mesenchymal stem cells. It contains 10X DMSO and does not use any animal-derived components. The product also contains phenol red. 

• Completed medium 1X

• No antibiotics - antimycotics

• No animal derived protein

• Quality and traceability guarantee by ISO 13485:2016

Cryosave II

Crosave II is supplied in a 1X format for the cryopreservation of cells and mesenchymal stem cells. It contains 5% DMSO and is manufactured with a special formula that includes all components that meet USP standards.
• Completed medium 1X
• No antibiotics - antimycotics
• No animal derived protein
• No phenol red
• Quality and traceability guarantee by ISO 13485:2016.

MSCCryosave OTS

MSCCryosave OTS is supplied in a 1X format for the cryopreservation of Mesenchymal stem cells. The product contains human serum albumin, which meets USP standards

• Completed medium 1X
• No antibiotics - antimycotics
• No animal derived protein
• No phenol red
• No cryoprotectant (DMSO, glycerol...)
• Quality and traceability guarantee by ISO 13485:2016

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the MSCCryosave OTS  at 2-8? and protected from light until ready to use.

Usages

MSCCryosave OTS is well suited for the production of off-the-shelf stem cell medicine. The product has been assessed for its effectiveness in cryopreservation – of mesenchymal stem cells from adipose tissue and human umbilical cord – for good cryopreservation efficiency with a high percentage of viable cells after defrosting.

 

ThawBest

Thawbest is a new solution to improve the cell viability during thawing process. Thawbest helps the reduction of shock/stress during the thawing by gradual decrease of osmolarity.

Thawbest is suitable for all kinds of cell cryopreservation media. It should be used with ratio 2:1 (2 vol of Thawbest : 1 vol of cell suspension).

 

AfterFreeze

Afterfreeze is a solution used to thaw off-the-shelf mesenchymal stem cells after freezing to increase the percentage of living cells compared to physiological saline. AfterFreeze is manufactured with ingredients that meet USP standards, according to the guidelines of GMP-WHO.

Usages

Using in the process of defrosting frozen cells. The product was evaluated on mesenchymal stem cells from human adipose tissue and umbilical cord.

 

Cell Extraction Mini Kit

Cell Extraction Mini Kit is a set of reagents and consumables used to extract single cells from solid tissues (up to 10 grams of tissue) such as adipose tissue, umbilical cord,...

The products were tested with high efficacy for adipose tissues and umbilical cord tissues to collect single cells.

To increase the extraction efficacy, this product uses the combitorial mechanisms of enzymes and machenic forces to break up the solid tissues to single cells.

The enzyme cocktails are specially desinged with high-puried collagenase with dispase.

Quality and traceability guarantee by ISO 13485:2016.

ADSCCult II

ADSCCult II is a product used to promote the growth of mesenchymal stem cells obtained from human adipose tissue. The product is formulated with unique ingredients that stimulate cellular self- renewal, inhibit cell differentiation, and prevent cell aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCult II

MSCCult II is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord tissue. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics - antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO 13485:2016.

 

BMMSCCult

BMMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics 

• Coating is NOT required

• With high-stable growth factors

• Quality and trace ability guarantee by ISO13485:2016

DentalCult

DentalCult is a product that promotes the growth of mesenchymal stem cells derived from human bone marrow. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCryosave OTS TH

MSCCryosave OTS TH is supplied in a 1X format for the cryopreservation of Mesenchymal stem cells. It contains human serum albumin, which meets USP standards, and trehalose.
• Completed medium 1X
• No antibiotics - antimycotics
• No animal derived protein
• No phenol red
• No cryoprotectant (DMSO, glycerol...)
• Quality and traceability guarantee by ISO 13485:2016

The products are for in vitro use only and not for diagnostic or therapeutic procedures.

Store the MSCCryosave OTS  at 2-8°C and protected from light until ready to use.

Usages

MSCCryosave OTS is well suited for the production of off-the-shelf stem cell medicine. The product has been assessed for its effectiveness in cryopreservation – of mesenchymal stem cells from adipose tissue and human umbilical cord – for good cryopreservation efficiency with a high percentage of viable cells after defrosting.

 

UCBMSCCult

UCBMSCCult is a product that promotes the growth of mesenchymal stem cells derived from human umbilical cord blood. It is formulated with unique ingredients that stimulate cellular self-renewal, inhibit differentiation, and prevent aging during in vitro expansion.

• Completed medium

• No antibiotics-antimycotics

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO13485:2016.

MSCCult OTS

MSCCult OTS is a product with an advanced formula that does not use phenol red or any animal-derived protein. It has low endotoxin and is suitable for promoting the growth of mesenchymal stem cells from different tissue types.

• Completed medium

• No antibiotics - antimycotics

• Coating is NOT required

• With high-stable growth factors

• Stem cell drug production pipelines

• Quality and traceability guarantee by ISO 13485:2016

 

MSCCult OTS MV

MSCCult OTS MV with low toxicity, it is suitable for producing conditioned medium, extracellular microvesicle, and exosomes from various types of mesenchymal stem cells.

• Completed medium

• No antibiotics - antimycotics

• No phenol red

• No animal-derived protein

• Coating is NOT required

• With high-stable growth factors

• Quality and traceability guarantee by ISO 13485:2016.

MSCCult CD

MSCCult CD is a product manufactured without any attachment factors and requires surface coating before cell seeding.

? Completed medium

• No antibiotics - antimycotics

• No phenol red

• No animal-derived protein

• With high-stable growth factors

• Coating required before seeding

• Quality and traceability guarantee by ISO 13485:2016.

ImmunoAdd

ImmunoAdd enhances the immune modulation characteristics of mesenchymal stem cells. The productis in the form of 100X and is added to the growth medium for mesenchymal stem cells. It contains components that significantly stimulate the immune modulation properties of mesenchymal stem cells after 48 hours of treatment.

• Completed medium

• No antibiotics - antimycotics

• Coating is NOT required

• Quality and traceability guarantee by ISO 13485:2016.

SecretoAdd

SecretoAdd enhances the secretion ability of mesenchymal stem cells. The product is produced in a 100X format and is directly added to the culture medium. After 48 hours of culture under low oxygen conditions (5-10%), the amount of secretion significantly increases.

• Completed medium 

• No antibiotics - antimycotics 

• Coating is NOT required 

• Quality and traceability guarantee by ISO 13485:2016.

MSCCult Muse

MSCCult Muse enhances the population of SSEA-3+CD105+ muse cells. The product is produced in a completed format and does not require the addition of any components prior to use. 

•  Completed medium 

• No antibiotics - antimycotics 

• Coating is NOT required 

• Quality and traceability guarantee by ISO 13485:2016.

Trypsinizer

Trypsinizer is used to detach mesenchymal stem cells when culturing them on a flask or dish. It utilizes a combination of plant-derived trypsin enzymes.
- Completed medium
- No antibiotics - antimycotics
- No animal derived protein
- No phenol red
- Quality and traceability guarantee by ISO 13485:2016.

 

Deattachment Advanced

Deattachment Advanced is used to detach mesenchymal stem cells when culturing them on flasks or in bioreactors. It utilizes a combination of recombinant trypsin and collagenase.
? Completed medium
? No antibiotics - antimycotics
? No animal derived protein
? No phenol red
? Quality and traceability guarantee by ISO 13485:2016

Phosphate Buffer Saline (PBS) OTS

PBS OTS is a product used for cell washing with low endotoxin levels, making it suitable for the production of therapeutic
cells.
? Completed medium
? No antibiotics - antimycotics
? No animal derived protein
? No phenol red
? Low endotoxin
? Quality and traceability guarantee by ISO 13485:2016